Hotspot

rpl23-rpl2

IRa · 173271-173288 · 18 bp

Back to Aquilaria

Pi 0.0505

MarkerSeek score 57.00

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 18
variable_sites: 3
pi: 0.0505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	GAATGGTTAAAGCGCCCAAC	CACTTGACTGTCTACGGCTC	50.0	55.0	58.0	58.0	598 bp	1.0	80.5
rpl23-rpl2_p2	GAATGGTTAAAGCGCCCAAC	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	645 bp	1.0	80.4
rpl23-rpl2_p3	GAATGGTTAAAGCGCCCAAC	TTCTGTCGGTGCTTGAAACA	50.0	45.0	58.0	58.0	1154 bp	1.0	80.0
rpl23-rpl2_p4	CCTGTTAAGCATCCATGGCT	CACTTGACTGTCTACGGCTC	50.0	55.0	57.9	58.0	618 bp	1.0	80.0
rpl23-rpl2_p5	GAATGGTTAAAGCGCCCAAC	TGTGAATAACCCGTCCTCCT	50.0	50.0	58.0	58.1	1200 bp	1.0	79.8