Hotspot

ndhF-rpl32

SSC · 131608-132414 · 807 bp

Back to Aquilaria

Pi 0.0148

MarkerSeek score 64.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 807
variable_sites: 39
pi: 0.0148
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACGAAAGGTACGATCCA	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.3	57.9	836-956 bp	1.0	81.2
ndhF-rpl32_p2	GGAACGAAAGGTACGATCCAT	AAAAGCTTTTAACGCTGCCC	47.6	45.0	57.8	57.9	836-956 bp	1.0	77.1
ndhF-rpl32_p3	GGAACGAAAGGTACGATCCA	AGCTTTTAACGCTGCCCAAT	50.0	45.0	57.3	58.7	833-953 bp	1.0	76.5
ndhF-rpl32_p4	AAGTGGAACGAAAGGTACGA	AAAAGCTTTTAACGCTGCCC	45.0	45.0	56.8	57.9	840-960 bp	0.909091	72.8
ndhF-rpl32_p5	AGTGGAACGAAAGGTACGATC	AAAAGCTTTTAACGCTGCCC	47.6	45.0	57.8	57.9	839-959 bp	0.909091	72.4