Hotspot

cemA-ycf4

LSC · 67126-68077 · 952 bp

Back to Aquilaria

Pi 0.00721

MarkerSeek score 53.30

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 24
pi: 0.00721
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	GAGTGAAATCCACCAAGGCA	TGACTCCGCGAGAAATTGAG	50.0	50.0	58.1	58.0	1063-1107 bp	1.0	84.3
cemA-ycf4_p2	AGAGTGAAATCCACCAAGGC	TGACTCCGCGAGAAATTGAG	50.0	50.0	57.8	58.0	1064-1108 bp	1.0	83.4
cemA-ycf4_p3	GAGTGAAATCCACCAAGGCA	AAAAAGCTGCTGAATTGGCC	50.0	45.0	58.1	57.8	1042-1086 bp	1.0	83.1
cemA-ycf4_p4	GAGTGAAATCCACCAAGGCA	TATTTCTTGCGCGTACCGAT	50.0	45.0	58.1	57.8	1022-1066 bp	1.0	82.9
cemA-ycf4_p5	GAGTGAAATCCACCAAGGCA	AAAGCTGCTGAATTGGCCTA	50.0	45.0	58.1	57.8	1040-1084 bp	1.0	82.7