Hotspot

rps4-trnT-UGU

LSC · 48845-49360 · 516 bp

Back to Aquilaria

Pi 0.018

MarkerSeek score 66.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 23
pi: 0.018
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	58.0	58.0	674-691 bp	1.0	85.3
rps4-trnT-UGU_p2	AGGTCCTCGATAACGTGACA	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	58.2	58.0	598-615 bp	1.0	84.9
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	659-676 bp	1.0	84.6
rps4-trnT-UGU_p4	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	627-644 bp	1.0	84.0
rps4-trnT-UGU_p5	CTCCCAGACGGCGTATTTTT	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	58.3	58.0	626-643 bp	1.0	83.8