Hotspot

trnG-UCC-trnfM-CAU

LSC · 38918-39125 · 208 bp

Back to Aquilaria

Pi 0.0119

MarkerSeek score 57.80

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 6
pi: 0.0119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCAAGAACGAACAATGCGGA	CGGGATAGAGCAGTCTGGTA	45.0	55.0	58.1	58.0	346-365 bp	1.0	84.4
trnG-UCC-trnfM-CAU_p2	TCAAGAACGAACAATGCGGA	TAGGGTTTCCTTTCATCGCG	45.0	50.0	58.1	58.0	364-383 bp	1.0	84.3
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	CGGGATAGAGCAGTCTGGTA	50.0	55.0	57.8	58.0	304-323 bp	1.0	84.0
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGGGTTTCCTTTCATCGCG	50.0	50.0	57.8	58.0	322-341 bp	1.0	83.9
trnG-UCC-trnfM-CAU_p5	TCAAGAACGAACAATGCGGA	CCTTTCATCGCGGGATAGAG	45.0	55.0	58.1	57.9	356-375 bp	1.0	83.9