Hotspot

psbM-trnD-GUC

LSC · 31557-32310 · 754 bp

Back to Aquilaria

Pi 0.00891

MarkerSeek score 46.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 17
pi: 0.00891
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGCCTTGGCTGATTGTTTTT	TATGTGTATGCTGCGCACTT	45.0	45.0	57.8	58.0	960-978 bp	1.0	80.2
psbM-trnD-GUC_p2	CGCCTTGGCTGATTGTTTTT	GTGTATGCTGCGCACTTTTT	45.0	45.0	57.8	57.9	957-975 bp	1.0	80.0
psbM-trnD-GUC_p3	ATGAACAGCGCAGTAGCAAT	TATGTGTATGCTGCGCACTT	45.0	45.0	58.3	58.0	904-922 bp	1.0	79.4
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	GTGTATGCTGCGCACTTTTT	45.0	45.0	58.3	57.9	901-919 bp	1.0	79.2
psbM-trnD-GUC_p5	CGCCTTGGCTGATTGTTTTT	ATATGTGTATGCTGCGCACT	45.0	45.0	57.8	57.8	961-979 bp	1.0	78.4