Hotspot

atpF-atpH

LSC · 13914-14446 · 533 bp

Back to Aquilaria

Pi 0.00362

MarkerSeek score 53.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 7
pi: 0.00362
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTTAACCCGAAACTCCCGG	TGGTCTGGTTGTAGCATTGG	50.0	50.0	57.9	57.8	649-657 bp	1.0	79.2
atpF-atpH_p2	GGCCAGTGACCTAAGGAAAC	TGGTCTGGTTGTAGCATTGG	55.0	50.0	58.2	57.8	624-632 bp	1.0	78.6
atpF-atpH_p3	ATGGCCAGTGACCTAAGGAA	TGGTCTGGTTGTAGCATTGG	50.0	50.0	58.3	57.8	626-634 bp	1.0	77.4
atpF-atpH_p4	TGGCCAGTGACCTAAGGAAA	TGGTCTGGTTGTAGCATTGG	50.0	50.0	58.6	57.8	625-633 bp	1.0	75.6
atpF-atpH_p5	GCCAGTGACCTAAGGAAACG	TGGTCTGGTTGTAGCATTGG	55.0	50.0	58.6	57.8	623-631 bp	1.0	75.6