Hotspot

ndhI

SSC · 118986-119525 · 540 bp

Back to Apostasia

Pi 0.4

MarkerSeek score 77.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 540
variable_sites: 216
pi: 0.4
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CAAGCTCGTACACATTGGGT	TGGAATAGCGATGGAAGCAG	50.0	50.0	58.2	57.8	2002 bp	0.5	56.5
ndhI_p2	CAAGCTCGTACACATTGGGT	GGAATAGCGATGGAAGCAGT	50.0	50.0	58.2	57.8	2001 bp	0.5	56.5
ndhI_p3	CAAGCTCGTACACATTGGGT	CGTCAGCCTATAGGGTTCCT	50.0	55.0	58.2	58.3	1886 bp	0.5	56.1
ndhI_p4	GCTCGTACACATTGGGTACA	TGGAATAGCGATGGAAGCAG	50.0	50.0	57.6	57.8	1999 bp	0.5	55.1
ndhI_p5	GCTCGTACACATTGGGTACA	GGAATAGCGATGGAAGCAGT	50.0	50.0	57.6	57.8	1998 bp	0.5	55.0