Hotspot

clpP

LSC · 69737-72399 · 2663 bp

Back to Apostasia

Pi 0.0234

MarkerSeek score 57.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2663
variable_sites: 61
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCGGGAGATTTCGTGAGATT	AGACAAAAGACGATGAAGTGA	50.0	38.1	58.1	55.2	2961-2976 bp	1.0	52.6
clpP_p2	TAATGGTTGGCATGTCGTGT	AGACAAAAGACGATGAAGTGA	45.0	38.1	57.8	55.2	2906-2921 bp	1.0	51.6
clpP_p3	TGGCTGTCTTGCGTTTCTAA	AGACAAAAGACGATGAAGTGA	45.0	38.1	57.8	55.2	2935-2950 bp	1.0	51.1
clpP_p4	TTGGCTGTCTTGCGTTTCTA	AGACAAAAGACGATGAAGTGA	45.0	38.1	57.8	55.2	2936-2951 bp	1.0	51.1
clpP_p5	GGTTGGCATGTCGTGTCTAT	AGACAAAAGACGATGAAGTGA	50.0	38.1	58.0	55.2	2902 bp	0.5	29.5