Hotspot

rps3-rpl22

LSC · 85814-85926 · 113 bp

Back to Apostasia

Pi 0.22

MarkerSeek score 79.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 18
pi: 0.22
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAAGTCGGAAACCAAGTGGA	GAAAAAGGTAAAAGCCCGAGC	50.0	47.6	58.0	58.1	218-244 bp	1.0	81.5
rps3-rpl22_p2	TCCAAGTCGGAAACCAAGTG	GAAAAAGGTAAAAGCCCGAGC	50.0	47.6	58.0	58.1	220-246 bp	1.0	81.4
rps3-rpl22_p3	CAAGTCGGAAACCAAGTGGA	AAAAAGGTAAAAGCCCGAGC	50.0	45.0	58.0	56.9	217-243 bp	1.0	81.1
rps3-rpl22_p4	TCCAAGTCGGAAACCAAGTG	AAAAAGGTAAAAGCCCGAGC	50.0	45.0	58.0	56.9	219-245 bp	1.0	81.1
rps3-rpl22_p5	CTCTGGGTTGTTCCAAGTCG	GAAAAAGGTAAAAGCCCGAGC	55.0	47.6	58.5	58.1	231-257 bp	1.0	77.5