Hotspot

clpP-psbB

LSC · 72400-73128 · 729 bp

Back to Apostasia

Pi 0.0516

MarkerSeek score 74.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 37
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	AGGCACTTTTGGAACACCAA	GATCAAGCACAGGGTCAGAG	45.0	55.0	58.1	58.0	916-960 bp	1.0	83.1
clpP-psbB_p2	AGGCACTTTTGGAACACCAA	ACAGGGTCAGAGGGATCAAA	45.0	50.0	58.1	58.0	908-952 bp	1.0	82.8
clpP-psbB_p3	AGGCACTTTTGGAACACCAA	CAGATAGCTGCCAAGAAGCA	45.0	50.0	58.1	58.0	1094-1138 bp	1.0	82.3
clpP-psbB_p4	AGGCACTTTTGGAACACCAA	AGATAGCTGCCAAGAAGCAC	45.0	50.0	58.1	58.0	1093-1137 bp	1.0	82.2
clpP-psbB_p5	AGGCACTTTTGGAACACCAA	ACACCCATATTCCAGGACCA	45.0	50.0	58.1	58.0	1261-1305 bp	1.0	81.9