Hotspot

atpA-trnR-UCU

LSC · 43621-43666 · 46 bp

Back to Apostasia

Pi 0.152

MarkerSeek score 75.90

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 46
variable_sites: 7
pi: 0.152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TCAGGAGCAGATCGAACTCT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.9	57.3	111-112 bp	1.0	70.4
atpA-trnR-UCU_p2	CCGAAGAAGCTGAAACCCTT	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.1	57.3	145-146 bp	1.0	69.5
atpA-trnR-UCU_p3	TCACCGAAGAAGCTGAAACC	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.1	57.3	148-149 bp	1.0	69.3
atpA-trnR-UCU_p4	CCGAAGAAGCTGAAACCCTT	GTTCAAATCCTATTGGACGCA	50.0	42.9	58.1	56.6	144-145 bp	1.0	63.9
atpA-trnR-UCU_p5	TCACCGAAGAAGCTGAAACC	GTTCAAATCCTATTGGACGCA	50.0	42.9	58.1	56.6	147-148 bp	1.0	63.7