primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score matK-rps16_p1 matK-rps16 1 TCGCGACGAGAATGGAATTT TCATTTTTGCTCGAGCCGTA 20 20 45.0 45.0 58.0 57.9 3.7 33.2 0.092084 3532 5780 2695-2870 1.000000 85.7 matK-rps16_p2 matK-rps16 2 TCGCGACGAGAATGGAATTT TTTTTGCTCGAGCCGTATGA 20 20 45.0 45.0 58.0 57.9 3.7 33.2 0.092084 3532 5780 2692-2867 1.000000 85.7 matK-rps16_p3 matK-rps16 3 TCGCGACGAGAATGGAATTT TCGATCCCGAAGAGAAGGAA 20 20 45.0 50.0 58.0 57.9 3.7 0.0 0.143906 3532 5780 2587-2763 1.000000 85.4 matK-rps16_p4 matK-rps16 4 TCGCGACGAGAATGGAATTT GGTGCTCAACCTACAGGAAC 20 20 45.0 55.0 58.0 58.2 3.7 1.3 0.194899 3532 5780 2481-2657 1.000000 85.1 matK-rps16_p5 matK-rps16 5 ACTAATCGCGACGAGAATGG TTTTTGCTCGAGCCGTATGA 20 20 50.0 45.0 57.9 57.9 22.2 33.2 0.208617 3532 5780 2697-2872 1.000000 84.8 rps16_p1 rps16 1 TGGAATTGCGCTGTACAACT CAAGTGGTAAGGCAATGGGT 20 20 45.0 50.0 58.1 58.1 9.7 0.0 0.185768 5781 7069 2666-2808 1.000000 84.0 rps16_p2 rps16 2 GGAATTGCGCTGTACAACTG CAAGTGGTAAGGCAATGGGT 20 20 50.0 50.0 57.8 58.1 9.7 0.0 0.323955 5781 7069 2665-2807 1.000000 82.8 rps16_p3 rps16 3 GAATTGCGCTGTACAACTGC CAAGTGGTAAGGCAATGGGT 20 20 50.0 50.0 58.4 58.1 9.7 0.0 0.470038 5781 7069 2664-2806 1.000000 81.7 rps16_p4 rps16 4 TGGAATTGCGCTGTACAACT GGGGCCCCATTTTTACTCTT 20 20 45.0 50.0 58.1 57.8 9.7 37.6 0.356823 5781 7069 2211-2211 0.500000 57.1 rps16_p5 rps16 5 GGAATTGCGCTGTACAACTG GGGGCCCCATTTTTACTCTT 20 20 50.0 50.0 57.8 57.8 9.7 37.6 0.495010 5781 7069 2210-2210 0.500000 56.0 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGTCGCACGTTGCTTTCTA CAAGTGGTAAGGCAATGGGT 20 20 45.0 50.0 58.1 58.1 8.1 0.0 0.215968 7070 7695 752-774 1.000000 84.2 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 20 20 45.0 50.0 58.1 57.3 8.1 16.9 0.806797 7070 7695 718-740 1.000000 79.8 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 TATCCTTCAAGTCGCACGTT CAAGTGGTAAGGCAATGGGT 20 20 45.0 50.0 57.3 58.1 0.0 0.0 0.812788 7070 7695 760-782 1.000000 79.4 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 AAGTCGCACGTTGCTTTCTA AAGTGGTAAGGCAATGGGTT 20 20 45.0 45.0 58.1 57.3 8.1 0.0 0.833940 7070 7695 751-773 1.000000 79.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 AAGTCGCACGTTGCTTTCTA AGTGGTAAGGCAATGGGTTT 20 20 45.0 45.0 58.1 57.3 8.1 0.0 0.833940 7070 7695 750-772 1.000000 79.3 psbK-psbI_p1 psbK-psbI 1 GTTTGGCAAGCTGCTGTAAG TTTTATTCCTCACGCCCAGG 20 20 50.0 50.0 57.9 57.9 1.4 0.0 0.178878 8236 8408 316-637 1.000000 80.4 psbK-psbI_p2 psbK-psbI 2 ATGCCCCTTTCAATCCCATC TTTTATTCCTCACGCCCAGG 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.215382 8236 8408 384-705 1.000000 79.9 psbK-psbI_p3 psbK-psbI 3 TTCACCAAATTACCCGAGGC TTTTATTCCTCACGCCCAGG 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.293804 8236 8408 406-727 1.000000 79.2 psbK-psbI_p4 psbK-psbI 4 CCCATCGTAGACGTTATGCC TTTTATTCCTCACGCCCAGG 20 20 55.0 50.0 58.2 57.9 1.5 0.0 0.343214 8236 8408 370-691 1.000000 78.9 psbK-psbI_p5 psbK-psbI 5 CACCAAATTACCCGAGGCAT TTTTATTCCTCACGCCCAGG 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.360076 8236 8408 404-725 1.000000 78.7 trnG-UCC-psbZ_p1 trnG-UCC-psbZ 1 AATCGAACCCGCATCTTCTC CTTCCGATGGTTGGTCAAGT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.243679 17410 17711 447-464 1.000000 83.5 trnG-UCC-psbZ_p2 trnG-UCC-psbZ 2 GAATCGAACCCGCATCTTCT CTTCCGATGGTTGGTCAAGT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.243679 17410 17711 448-465 1.000000 83.5 trnG-UCC-psbZ_p3 trnG-UCC-psbZ 3 ACATTACTTCACCATGGGGC CTTCCGATGGTTGGTCAAGT 20 20 50.0 50.0 57.9 57.8 18.1 0.0 0.328880 17410 17711 477-501 1.000000 82.8 trnG-UCC-psbZ_p4 trnG-UCC-psbZ 4 AATCGAACCCGCATCTTCTC TTGCTTCTTCCGATGGTTGG 20 20 50.0 50.0 58.1 58.5 0.0 0.0 0.525050 17410 17711 453-470 1.000000 81.2 trnG-UCC-psbZ_p5 trnG-UCC-psbZ 5 GAATCGAACCCGCATCTTCT TTGCTTCTTCCGATGGTTGG 20 20 50.0 50.0 58.1 58.5 0.0 0.0 0.525050 17410 17711 454-471 1.000000 81.2 rpoC1-rpoC2_p1 rpoC1-rpoC2 1 TGATGGGGATCAAATGGCTG CCGCGTGTACATTCCCATAA 20 20 50.0 50.0 57.9 58.1 0.0 4.9 0.133530 33110 33350 1084-1086 1.000000 83.4 rpoC1-rpoC2_p2 rpoC1-rpoC2 2 GATGGGGATCAAATGGCTGT CCGCGTGTACATTCCCATAA 20 20 50.0 50.0 57.9 58.1 0.0 4.9 0.135010 33110 33350 1083-1085 1.000000 83.3 rpoC1-rpoC2_p3 rpoC1-rpoC2 3 TGATGGGGATCAAATGGCTG TCTAGCTCCCGAAAAGGACA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.143186 33110 33350 1215-1217 1.000000 82.8 rpoC1-rpoC2_p4 rpoC1-rpoC2 4 TGATGGGGATCAAATGGCTG GTCTACGGGTGAGGTATCCA 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.139178 33110 33350 1420-1422 1.000000 82.3 rpoC1-rpoC2_p5 rpoC1-rpoC2 5 GATGGGGATCAAATGGCTGT GTCTACGGGTGAGGTATCCA 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.140658 33110 33350 1419-1421 1.000000 82.3 atpI-atpH_p1 atpI-atpH 1 AACTTTGGCTGCGGCTTATA AGCCAATCCAGCAGCAATAA 20 20 45.0 45.0 57.9 57.9 18.8 0.0 0.271229 39506 40247 823-839 1.000000 82.2 atpI-atpH_p2 atpI-atpH 2 AACTTTGGCTGCGGCTTATA CAGCAGCAATAACAGAAGCG 20 20 45.0 50.0 57.9 57.8 18.8 0.0 0.292446 39506 40247 815-831 1.000000 82.0 atpI-atpH_p3 atpI-atpH 3 AACTTTGGCTGCGGCTTATA GCAGCAGTACCTTGACCAAT 20 20 45.0 50.0 57.9 58.2 18.8 0.0 0.296398 39506 40247 870-886 1.000000 81.7 atpI-atpH_p4 atpI-atpH 4 AACTTTGGCTGCGGCTTATA GCCAATCCAGCAGCAATAAC 20 20 45.0 50.0 57.9 57.8 18.8 0.0 0.337170 39506 40247 822-838 1.000000 81.6 atpI-atpH_p5 atpI-atpH 5 AACTTTGGCTGCGGCTTATA GCGGCAGAAATAAGTGGACT 20 20 45.0 50.0 57.9 58.3 18.8 0.0 0.383717 39506 40247 798-814 1.000000 81.4 atpA-trnR-UCU_p1 atpA-trnR-UCU 1 TCAGGAGCAGATCGAACTCT GGTTCAAATCCTATTGGACGC 20 21 50.0 47.6 57.9 57.3 11.0 0.0 1.829089 43621 43666 111-112 1.000000 70.4 atpA-trnR-UCU_p2 atpA-trnR-UCU 2 CCGAAGAAGCTGAAACCCTT GGTTCAAATCCTATTGGACGC 20 21 50.0 47.6 58.1 57.3 0.0 0.0 1.795231 43621 43666 145-146 1.000000 69.5 atpA-trnR-UCU_p3 atpA-trnR-UCU 3 TCACCGAAGAAGCTGAAACC GGTTCAAATCCTATTGGACGC 20 21 50.0 47.6 58.1 57.3 0.0 0.0 1.813838 43621 43666 148-149 1.000000 69.3 atpA-trnR-UCU_p4 atpA-trnR-UCU 4 CCGAAGAAGCTGAAACCCTT GTTCAAATCCTATTGGACGCA 20 21 50.0 42.9 58.1 56.6 0.0 0.0 2.498175 43621 43666 144-145 1.000000 63.9 atpA-trnR-UCU_p5 atpA-trnR-UCU 5 TCACCGAAGAAGCTGAAACC GTTCAAATCCTATTGGACGCA 20 21 50.0 42.9 58.1 56.6 0.0 0.0 2.516781 43621 43666 147-148 1.000000 63.7 rps4-trnT-UGU_p1 rps4-trnT-UGU 1 CCCAGACGGCGTATCTTTTT CTCAGAGGTTAGAGCATCGC 20 20 50.0 55.0 58.3 57.9 14.7 0.0 0.398577 46207 46590 468-494 1.000000 83.8 rps4-trnT-UGU_p2 rps4-trnT-UGU 2 CCCAGACGGCGTATCTTTTT GGTTCGATTCCGATAGCTGG 20 20 50.0 55.0 58.3 58.2 14.7 0.0 0.481312 46207 46590 427-453 1.000000 83.4 rps4-trnT-UGU_p3 rps4-trnT-UGU 3 GTTAATCCCGGTAAAGCCCC CTCAGAGGTTAGAGCATCGC 20 20 55.0 55.0 58.3 57.9 0.0 0.0 0.448797 46207 46590 486-512 1.000000 83.3 rps4-trnT-UGU_p4 rps4-trnT-UGU 4 CCCAGACGGCGTATCTTTTT ATGGTCATCGGTTCGATTCC 20 20 50.0 50.0 58.3 57.8 14.7 0.0 0.506331 46207 46590 436-462 1.000000 83.2 rps4-trnT-UGU_p5 rps4-trnT-UGU 5 GTTAATCCCGGTAAAGCCCC GGTTCGATTCCGATAGCTGG 20 20 55.0 55.0 58.3 58.2 0.0 0.0 0.531532 46207 46590 445-471 1.000000 82.9 psbL-psbF_p1 psbL-psbF 1 CATTCGGGTTTGATTGTGTCA AGTACGATGGTTGGCTGTTC 21 20 42.9 50.0 57.1 57.9 0.0 0.0 2.008310 64739 64760 132-132 1.000000 64.5 psbL-psbF_p2 psbL-psbF 2 CATTCGGGTTTGATTGTGTCA CAGTACGATGGTTGGCTGTT 21 20 42.9 50.0 57.1 58.2 0.0 0.0 2.105137 64739 64760 133-133 1.000000 63.8 psbL-psbF_p3 psbL-psbF 3 CATTCGGGTTTGATTGTGTCA GTACGATGGTTGGCTGTTCA 21 20 42.9 50.0 57.1 58.2 0.0 0.0 2.110214 64739 64760 131-131 1.000000 63.7 psbL-psbF_p4 psbL-psbF 4 CATTCGGGTTTGATTGTGTCA TACGATGGTTGGCTGTTCAC 21 20 42.9 50.0 57.1 58.2 0.0 0.0 2.110214 64739 64760 130-130 1.000000 63.7 psbL-psbF_p5 psbL-psbF 5 TCATTCGGGTTTGATTGTGTC AGTACGATGGTTGGCTGTTC 21 20 42.9 50.0 56.8 57.9 0.0 0.0 2.282699 64739 64760 133-133 1.000000 62.3 clpP_p1 clpP 1 GCGGGAGATTTCGTGAGATT AGACAAAAGACGATGAAGTGA 20 21 50.0 38.1 58.1 55.2 0.0 0.0 3.852813 69737 72399 2961-2976 1.000000 52.6 clpP_p2 clpP 2 TAATGGTTGGCATGTCGTGT AGACAAAAGACGATGAAGTGA 20 21 45.0 38.1 57.8 55.2 0.0 0.0 3.986802 69737 72399 2906-2921 1.000000 51.6 clpP_p3 clpP 3 TGGCTGTCTTGCGTTTCTAA AGACAAAAGACGATGAAGTGA 20 21 45.0 38.1 57.8 55.2 0.0 0.0 4.042418 69737 72399 2935-2950 1.000000 51.1 clpP_p4 clpP 4 TTGGCTGTCTTGCGTTTCTA AGACAAAAGACGATGAAGTGA 20 21 45.0 38.1 57.8 55.2 0.0 0.0 4.042418 69737 72399 2936-2951 1.000000 51.1 clpP_p5 clpP 5 GGTTGGCATGTCGTGTCTAT AGACAAAAGACGATGAAGTGA 20 21 50.0 38.1 58.0 55.2 0.0 0.0 3.818314 69737 72399 2902-2902 0.500000 29.5 clpP-psbB_p1 clpP-psbB 1 AGGCACTTTTGGAACACCAA GATCAAGCACAGGGTCAGAG 20 20 45.0 55.0 58.1 58.0 0.0 0.0 0.162053 72400 73128 916-960 1.000000 83.1 clpP-psbB_p2 clpP-psbB 2 AGGCACTTTTGGAACACCAA ACAGGGTCAGAGGGATCAAA 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.185491 72400 73128 908-952 1.000000 82.8 clpP-psbB_p3 clpP-psbB 3 AGGCACTTTTGGAACACCAA CAGATAGCTGCCAAGAAGCA 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.174802 72400 73128 1094-1138 1.000000 82.3 clpP-psbB_p4 clpP-psbB 4 AGGCACTTTTGGAACACCAA AGATAGCTGCCAAGAAGCAC 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.176228 72400 73128 1093-1137 1.000000 82.2 clpP-psbB_p5 clpP-psbB 5 AGGCACTTTTGGAACACCAA ACACCCATATTCCAGGACCA 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.160889 72400 73128 1261-1305 1.000000 81.9 psbB-psbT_p1 psbB-psbT 1 GTGCCAGAACCTTGTTCAGA TTAGTTGGAACTTTCGGCGG 20 20 50.0 50.0 58.0 58.5 9.4 7.2 0.517600 74656 75940 1467-1499 1.000000 83.7 psbB-psbT_p2 psbB-psbT 2 TGCCAGAACCTTGTTCAGAG TTAGTTGGAACTTTCGGCGG 20 20 50.0 50.0 57.7 58.5 9.4 7.2 0.745762 74656 75940 1466-1498 1.000000 81.9 psbB-psbT_p3 psbB-psbT 3 TCAGAGATGTTTTTGCCGGT TTAGTTGGAACTTTCGGCGG 20 20 45.0 50.0 57.7 58.5 1.9 7.2 0.755271 74656 75940 1452-1484 1.000000 81.8 psbB-psbT_p4 psbB-psbT 4 GGGACCCAACTACAAGGAGA TTAGTTGGAACTTTCGGCGG 20 20 55.0 50.0 58.3 58.5 5.3 7.2 0.831972 74656 75940 1380-1412 1.000000 81.4 psbB-psbT_p5 psbB-psbT 5 GCCAGAACCTTGTTCAGAGA TTAGTTGGAACTTTCGGCGG 20 20 50.0 50.0 57.4 58.5 9.4 7.2 1.035749 74656 75940 1465-1497 1.000000 79.5 psbT-psbN_p1 psbT-psbN 1 CCGCCGAAAGTTCCAACTAA GGGTACGCCTTATATACCGC 20 20 50.0 55.0 58.5 57.7 0.0 0.0 0.769181 76049 76114 174-174 1.000000 77.1 psbT-psbN_p2 psbT-psbN 2 CCGCCGAAAGTTCCAACTAA TCGCCATCTCTATATCGGGT 20 20 50.0 50.0 58.5 57.5 0.0 0.0 0.994085 76049 76114 212-212 1.000000 74.7 psbT-psbN_p3 psbT-psbN 3 TTTTTCGAGAACCGCCGAA GGGTACGCCTTATATACCGC 19 20 47.4 55.0 58.0 57.7 0.0 0.0 1.295067 76049 76114 185-185 1.000000 72.7 psbT-psbN_p4 psbT-psbN 4 CCGCCGAAAGTTCCAACTAA GTCGCCATCTCTATATCGGG 20 20 50.0 55.0 58.5 57.1 0.0 0.0 1.338650 76049 76114 213-213 1.000000 71.9 psbT-psbN_p5 psbT-psbN 5 CCGCCGAAAGTTCCAACTAA GGGGTACGCCTTATATACCG 20 20 50.0 55.0 58.5 57.1 0.0 21.2 1.432367 76049 76114 175-175 1.000000 71.7 petD_p1 petD 1 AGACATGTTGTTTGGATACTTCTC TGATGGTTGAATCAATACTTCTGT 24 24 37.5 33.3 56.9 56.4 0.0 0.0 10.695640 78356 79903 1664-1664 0.500000 20.0 petD_p2 petD 2 GACATGTTGTTTGGATACTTCTCT TGATGGTTGAATCAATACTTCTGT 24 24 37.5 33.3 56.9 56.4 0.0 0.0 10.695640 78356 79903 1663-1663 0.500000 20.0 petD_p3 petD 3 ACATGTTGTTTGGATACTTCTCT TGATGGTTGAATCAATACTTCTGT 23 24 34.8 33.3 55.9 56.4 0.0 0.0 10.793090 78356 79903 1662-1662 0.500000 20.0 petD_p4 petD 4 AGACATGTTGTTTGGATACTTCT TGATGGTTGAATCAATACTTCTGT 23 24 34.8 33.3 55.9 56.4 0.0 0.0 10.793090 78356 79903 1664-1664 0.500000 20.0 petD_p5 petD 5 AGACATGTTGTTTGGATACTTCTCT TGATGGTTGAATCAATACTTCTGT 25 24 36.0 33.3 58.2 56.4 0.0 0.0 10.870254 78356 79903 1664-1664 0.500000 20.0 rps3-rpl22_p1 rps3-rpl22 1 CAAGTCGGAAACCAAGTGGA GAAAAAGGTAAAAGCCCGAGC 20 21 50.0 47.6 58.0 58.1 3.8 0.0 1.095497 85814 85926 218-244 1.000000 81.5 rps3-rpl22_p2 rps3-rpl22 2 TCCAAGTCGGAAACCAAGTG GAAAAAGGTAAAAGCCCGAGC 20 21 50.0 47.6 58.0 58.1 0.0 0.0 1.095497 85814 85926 220-246 1.000000 81.4 rps3-rpl22_p3 rps3-rpl22 3 CAAGTCGGAAACCAAGTGGA AAAAAGGTAAAAGCCCGAGC 20 20 50.0 45.0 58.0 56.9 3.8 0.0 1.138970 85814 85926 217-243 1.000000 81.1 rps3-rpl22_p4 rps3-rpl22 4 TCCAAGTCGGAAACCAAGTG AAAAAGGTAAAAGCCCGAGC 20 20 50.0 45.0 58.0 56.9 0.0 0.0 1.138970 85814 85926 219-245 1.000000 81.1 rps3-rpl22_p5 rps3-rpl22 5 CTCTGGGTTGTTCCAAGTCG GAAAAAGGTAAAAGCCCGAGC 20 21 55.0 47.6 58.5 58.1 0.0 0.0 1.544364 85814 85926 231-257 1.000000 77.5 ycf1_p1 ycf1 1 GAGCTACTGAGGAACAACGG ACTCGACACCATTGTGCTTT 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.035012 112547 113688 2249-2249 0.500000 59.7 ycf1_p2 ycf1 2 GCTTCCTTCGTAACTCCAGG ACTCGACACCATTGTGCTTT 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.044222 112547 113688 2169-2169 0.500000 59.6 ycf1_p3 ycf1 3 AAAGCGAGCCCTTTCATTCA ACTCGACACCATTGTGCTTT 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.122982 112547 113688 2361-2361 0.500000 59.0 ycf1_p4 ycf1 4 AAGCGAGCCCTTTCATTCAA ACTCGACACCATTGTGCTTT 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.122982 112547 113688 2360-2360 0.500000 59.0 ycf1_p5 ycf1 5 TCAAAGCGAGCCCTTTCATT ACTCGACACCATTGTGCTTT 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.122982 112547 113688 2363-2363 0.500000 59.0 ndhI_p1 ndhI 1 CAAGCTCGTACACATTGGGT TGGAATAGCGATGGAAGCAG 20 20 50.0 50.0 58.2 57.8 0.0 0.0 0.436749 118986 119525 2002-2002 0.500000 56.5 ndhI_p2 ndhI 2 CAAGCTCGTACACATTGGGT GGAATAGCGATGGAAGCAGT 20 20 50.0 50.0 58.2 57.8 0.0 0.0 0.438308 118986 119525 2001-2001 0.500000 56.5 ndhI_p3 ndhI 3 CAAGCTCGTACACATTGGGT CGTCAGCCTATAGGGTTCCT 20 20 50.0 55.0 58.2 58.3 0.0 21.0 0.485717 118986 119525 1886-1886 0.500000 56.1 ndhI_p4 ndhI 4 GCTCGTACACATTGGGTACA TGGAATAGCGATGGAAGCAG 20 20 50.0 50.0 57.6 57.8 24.8 0.0 0.618700 118986 119525 1999-1999 0.500000 55.1 ndhI_p5 ndhI 5 GCTCGTACACATTGGGTACA GGAATAGCGATGGAAGCAGT 20 20 50.0 50.0 57.6 57.8 24.8 0.0 0.620259 118986 119525 1998-1998 0.500000 55.0