Hotspot

ycf1

SSC · 124737-130244 · 5508 bp

Back to Aponogeton

Pi 0.0227

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5508
variable_sites: 186
pi: 0.0227
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTCGTTCTTGCTCTTGT	ATCTTCCTTGTCCCAAGCAT	45.0	45.0	58.0	56.8	1285-1300 bp	1.0	72.8
ycf1_p2	CTCTGCTTCGTTCTTGCTCT	ATCTTCCTTGTCCCAAGCAT	50.0	45.0	57.9	56.8	1288-1303 bp	1.0	72.7
ycf1_p3	AAAATCCACTTGGCTGGGTT	ATCTTCCTTGTCCCAAGCAT	45.0	45.0	57.9	56.8	1174-1189 bp	1.0	72.5
ycf1_p4	TAGCTCTGCTTCGTTCTTGC	ATCTTCCTTGTCCCAAGCAT	50.0	45.0	58.3	56.8	1291-1306 bp	1.0	71.0
ycf1_p5	TGCTTCGTTCTTGCTCTTGT	TCCTTGTCCCAAGCATATGT	45.0	45.0	58.0	56.5	1281-1296 bp	1.0	70.6