Hotspot

ndhF-rpl32

SSC · 112715-113925 · 1211 bp

Back to Aponogeton

Pi 0.0254

MarkerSeek score 64.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1211
variable_sites: 45
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAATTGGTCGTGTGAAC	TACACCTGGAATCGTCGAGA	50.0	50.0	58.0	57.9	2407-2476 bp	1.0	83.6
ndhF-rpl32_p2	AATACGAGCACATTCCCACC	TACACCTGGAATCGTCGAGA	50.0	50.0	58.0	57.9	2371-2440 bp	1.0	83.6
ndhF-rpl32_p3	CACATTCCCACCAATTCCCA	TACACCTGGAATCGTCGAGA	50.0	50.0	58.1	57.9	2363-2432 bp	1.0	83.4
ndhF-rpl32_p4	ATCCAAAGTCCCCTACACGA	TACACCTGGAATCGTCGAGA	50.0	50.0	58.1	57.9	2458-2527 bp	1.0	83.3
ndhF-rpl32_p5	TCCAAAGTCCCCTACACGAT	TACACCTGGAATCGTCGAGA	50.0	50.0	58.1	57.9	2457-2526 bp	1.0	83.3