Hotspot

trnF-GAA-ndhJ

LSC · 49089-49781 · 693 bp

Back to Aponogeton

Pi 0.0269

MarkerSeek score 66.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 27
pi: 0.0269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	ACAGAAACGTAGGTCCTCCA	ACGCTCTCAGTGTGCTTATG	50.0	50.0	58.0	58.0	1104-1139 bp	1.0	84.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACGCTCTCAGTGTGCTTATG	50.0	50.0	58.1	58.0	1018-1053 bp	1.0	84.6
trnF-GAA-ndhJ_p3	ACAGAAACGTAGGTCCTCCA	GTCGTTTATCCGCTTGGCTA	50.0	50.0	58.0	58.1	1249-1284 bp	1.0	83.8
trnF-GAA-ndhJ_p4	GGTAGATACGAGACACGCAC	ACGCTCTCAGTGTGCTTATG	55.0	50.0	57.9	58.0	1292-1330 bp	1.0	83.7
trnF-GAA-ndhJ_p5	ACAGAAACGTAGGTCCTCCA	CGTTTATCCGCTTGGCTAGT	50.0	50.0	58.0	58.1	1247-1281 bp	0.666667	69.9