Hotspot

trnS-UGA-psbZ

LSC · 36464-36818 · 355 bp

Back to Aponogeton

Pi 0.0254

MarkerSeek score 61.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 13
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	GGGAAGCAAATACAACGGGT	55.0	50.0	58.1	58.5	479-480 bp	1.0	78.0
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	GGGAAGCAAATACAACGGGT	55.0	50.0	58.1	58.5	468-469 bp	1.0	77.5
trnS-UGA-psbZ_p3	CAAGACCGGAGCTATCAACC	ACAACGGGTACGCTAATCAA	55.0	45.0	58.1	57.3	468-469 bp	1.0	75.8
trnS-UGA-psbZ_p4	AGCAAAAGGAGAGAGAGGGA	GGGAAGCAAATACAACGGGT	50.0	50.0	57.7	58.5	542-543 bp	1.0	75.6
trnS-UGA-psbZ_p5	CTATCAACCACTCGGCCATC	ACAACGGGTACGCTAATCAA	55.0	45.0	58.1	57.3	457-458 bp	1.0	75.3