Hotspot

psbC-trnS-UGA

LSC · 36244-36370 · 127 bp

Back to Aponogeton

Pi 0.0907

MarkerSeek score 82.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 17
pi: 0.0907
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	231-234 bp	1.0	85.1
psbC-trnS-UGA_p2	TCATTTATGGCATGCGGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.9	58.1	304-307 bp	1.0	84.3
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	242-245 bp	1.0	84.3
psbC-trnS-UGA_p4	TCATTTATGGCATGCGGGAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.9	58.2	250-253 bp	1.0	84.1
psbC-trnS-UGA_p5	TCATTTATGGCATGCGGGAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	57.9	58.1	315-318 bp	1.0	83.5