Hotspot

atpH-atpI

LSC · 14642-15444 · 803 bp

Back to Aponogeton

Pi 0.0399

MarkerSeek score 73.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 48
pi: 0.0399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	891-906 bp	1.0	80.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	864-879 bp	1.0	79.9
atpH-atpI_p3	AACGGAAGCGGCAGAAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	889-904 bp	1.0	79.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	892-907 bp	1.0	79.1
atpH-atpI_p5	AACGGAAGCGGCAGAAATAA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.2	57.4	862-877 bp	1.0	78.7