Hotspot

psbK-psbI

LSC · 8404-8662 · 259 bp

Back to Aponogeton

Pi 0.0425

MarkerSeek score 69.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 16
pi: 0.0425
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.6	311-313 bp	1.0	48.8
psbK-psbI_p2	AATCGTAGACGTTATGCCCG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.6	363-365 bp	1.0	47.6
psbK-psbI_p3	CGTTATGCCCGTCATACCTC	ACAAAGAGTTTGAGAGTAAGCA	55.0	36.4	58.2	55.6	354-356 bp	1.0	46.9
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	308-310 bp	1.0	46.8
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	309-311 bp	1.0	46.8