Hotspot

trnK-UUU-rps16

LSC · 4384-5116 · 733 bp

Back to Aponogeton

Pi 0.0332
MarkerSeek score 68.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
733
variable_sites
35
pi
0.0332
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ATCCGCTCACAGGAAAATCC TATCCCAATGGGCCGTCTAT 50.0 50.0 58.0 58.0 1398-1417 bp 0.666667 70.0
trnK-UUU-rps16_p2 ATCCGCTCACAGGAAAATCC TATCTATCCCAATGGGCCGT 50.0 50.0 58.0 58.0 1402-1421 bp 0.666667 70.0
trnK-UUU-rps16_p3 ACACATCCATCTCCACCTCA TATCCCAATGGGCCGTCTAT 50.0 50.0 58.0 58.0 1452-1455 bp 0.666667 68.0
trnK-UUU-rps16_p4 ACACATCCATCTCCACCTCA TATCTATCCCAATGGGCCGT 50.0 50.0 58.0 58.0 1456-1459 bp 0.666667 68.0
trnK-UUU-rps16_p5 ACACATCCATCTCCACCTCA CAATTGATGCTCGATCCCGA 50.0 50.0 58.0 58.1 1422-1425 bp 0.666667 67.3