Hotspot

rpoC2

LSC · 14041-18243 · 4203 bp

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Pi 0.00262

MarkerSeek score 55.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4203
variable_sites: 11
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGCACGATTGGAGTGGAATA	GCGCGCATTCTATTTCTGTC	50.0	50.0	58.1	58.0	218-243 bp	1.0	84.6
rpoC2_p2	TCATTCTCGCACGATTGGAG	GCGCGCATTCTATTTCTGTC	50.0	50.0	58.1	58.0	225-250 bp	1.0	84.4
rpoC2_p3	CGCACGATTGGAGTGGAATA	GTCTCCCTTTCGCTTCACAA	50.0	50.0	58.1	58.1	280-322 bp	1.0	82.5
rpoC2_p4	CGCACGATTGGAGTGGAATA	GATCCAGTCCAGTCTCCCTT	50.0	55.0	58.1	58.1	291-333 bp	1.0	82.3
rpoC2_p5	TCATTCTCGCACGATTGGAG	GTCTCCCTTTCGCTTCACAA	50.0	50.0	58.1	58.1	287-329 bp	1.0	82.3