Hotspot

psbA

LSC · 149471-150532 · 1062 bp

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Pi 0.453

MarkerSeek score 61.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 24
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCCTATACACCAAGACCGGG	ATAGAGAGTGTGTGCGCTTG	55.0	50.0	58.2	58.0	1142 bp	1.0	83.9
psbA_p2	TCCTATACACCAAGACCGGG	AAATAGAGAGTGTGTGCGCT	55.0	45.0	58.2	57.2	1144 bp	1.0	77.9
psbA_p3	TCCTATACACCAAGACCGGG	AATAGAGAGTGTGTGCGCTT	55.0	45.0	58.2	57.2	1143 bp	1.0	77.9
psbA_p4	TATACACCAAGACCGGGGG	ATAGAGAGTGTGTGCGCTTG	57.9	50.0	58.1	58.0	1139 bp	1.0	77.0
psbA_p5	CTATACACCAAGACCGGGGG	ATAGAGAGTGTGTGCGCTTG	60.0	50.0	59.2	58.0	1140 bp	1.0	75.7