Hotspot

rpl23-rpl2

LSC · 147542-147559 · 18 bp

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Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: LSC
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGCTTCAACCGGGTTATTCT	ACTGTCTACGGTTCCATTGC	50.0	50.0	58.3	57.9	128 bp	1.0	77.7
rpl23-rpl2_p2	CGCTTCAACCGGGTTATTCT	TGCTCGGGGTAGAAGTTTTG	50.0	50.0	58.3	57.8	105 bp	1.0	77.2
rpl23-rpl2_p3	ATCATTACGCTTCAACCGGG	ACTGTCTACGGTTCCATTGC	50.0	50.0	58.3	57.9	135 bp	1.0	77.1
rpl23-rpl2_p4	TCCGGGAAAGGGTAGAAGAA	ACTGTCTACGGTTCCATTGC	50.0	50.0	57.7	57.9	196 bp	1.0	77.0
rpl23-rpl2_p5	TTCCGGGAAAGGGTAGAAGA	ACTGTCTACGGTTCCATTGC	50.0	50.0	57.7	57.9	197 bp	1.0	77.0