Hotspot

trnL-CAA-ycf2

LSC · 139347-140065 · 719 bp

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Pi 0.00695

MarkerSeek score 62.70

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 5
pi: 0.00695
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	828 bp	1.0	78.6
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	827 bp	1.0	78.6
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	830 bp	1.0	78.6
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	829 bp	1.0	78.6
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	50.0	50.0	58.0	58.2	831 bp	1.0	78.6