Hotspot

rpl32-trnL-UAG

SSC · 109154-109613 · 460 bp

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Pi 0.013

MarkerSeek score 60.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 460
variable_sites: 6
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAGGGATATTGGACAGCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.4	57.9	596-598 bp	1.0	74.8
rpl32-trnL-UAG_p2	GAAGGGATATTGGACAGCGT	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.4	57.8	589-591 bp	1.0	74.4
rpl32-trnL-UAG_p3	GAAGGGATATTGGACAGCGT	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.4	57.8	595-597 bp	1.0	74.3
rpl32-trnL-UAG_p4	GAAGGGATATTGGACAGCGT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.4	57.8	594-596 bp	1.0	74.3
rpl32-trnL-UAG_p5	GAAGGGATATTGGACAGCGT	AAGATGACATGCCGCTACTC	50.0	50.0	57.4	57.8	646-648 bp	1.0	73.9