Hotspot

petA-psbJ

LSC · 60269-60962 · 694 bp

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Pi 0.00582

MarkerSeek score 63.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 4
pi: 0.00582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	835-839 bp	1.0	77.8
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	832-836 bp	1.0	76.7
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	830-834 bp	1.0	75.3
petA-psbJ_p4	ACGTGTCCAAGGCCTTTTAT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	828-832 bp	1.0	73.5
petA-psbJ_p5	ATTACGTGTCCAAGGCCTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	831-835 bp	1.0	73.5