Hotspot

ycf4-cemA

LSC · 57753-58384 · 632 bp

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Pi 0.00949

MarkerSeek score 64.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 6
pi: 0.00949
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	58.1	57.9	739-746 bp	1.0	79.0
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	753-760 bp	1.0	78.4
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	751-758 bp	1.0	78.0
ycf4-cemA_p4	AAGCTGCTGAATTGGCCTAT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.6	57.9	750-757 bp	1.0	76.2
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.6	57.9	749-756 bp	1.0	76.2