Hotspot

ndhK-ndhC

LSC · 47874-47920 · 47 bp

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Pi 0.0213

MarkerSeek score 64.70

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 47
variable_sites: 1
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCATAGAGAAGCGGCCATA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.3	57.8	206 bp	1.0	76.5
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.3	57.8	287 bp	1.0	76.4
ndhK-ndhC_p3	ACCATAGAGAAGCGGCCATA	GGGGCATTGGAATGGTCTTA	50.0	50.0	58.3	57.5	188 bp	1.0	74.5
ndhK-ndhC_p4	CCATAGAGAAGCGGCCATAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	57.2	57.8	286 bp	1.0	72.0
ndhK-ndhC_p5	CCATAGAGAAGCGGCCATAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.2	57.8	205 bp	1.0	72.0