Hotspot

ycf3-trnS-GGA

LSC · 42474-42947 · 474 bp

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Pi 0.0169

MarkerSeek score 63.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 8
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTCCCTTTCTCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	57.9	58.1	643-729 bp	1.0	79.6
ycf3-trnS-GGA_p2	CTCCCTTTCTCCCGAAGTTG	TCGGAAAGAGAGGGATTCGA	55.0	50.0	57.9	57.9	664-750 bp	1.0	79.2
ycf3-trnS-GGA_p3	CCCTTTCTCCCGAAGTTGTC	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.2	58.1	641-727 bp	1.0	78.9
ycf3-trnS-GGA_p4	CTCCCTTTCTCCCGAAGTTG	TTCCAATGCTACGCCTTGAA	55.0	45.0	57.9	57.8	614-700 bp	1.0	78.9
ycf3-trnS-GGA_p5	CCCTTTCTCCCGAAGTTGTC	TCGGAAAGAGAGGGATTCGA	55.0	50.0	58.2	57.9	662-748 bp	1.0	78.5