Hotspot

trnE-UUC-trnT-GGU

LSC · 28977-29605 · 629 bp

Back to Apocynum

Pi 0.0116
MarkerSeek score 69.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
629
variable_sites
7
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CCTATTACCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.0 58.0 767-931 bp 1.0 82.8
trnE-UUC-trnT-GGU_p2 CCTATTACCCTACCCCCAGG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.0 57.9 732-896 bp 1.0 82.2
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 733-897 bp 1.0 81.9
trnE-UUC-trnT-GGU_p4 CCTATTACCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.0 58.2 764-928 bp 1.0 81.7
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 698-862 bp 1.0 81.3