Hotspot

trnR-UCU-atpA

LSC · 7729-7868 · 140 bp

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Pi 0.0216

MarkerSeek score 64.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 3
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAATGAAAAGCGTCCA	AGACGTTTACCGAAGAAGCG	45.0	50.0	57.9	58.3	304-305 bp	1.0	77.7
trnR-UCU-atpA_p2	TCGGAATGAAAAGCGTCCAT	AGACGTTTACCGAAGAAGCG	45.0	50.0	57.9	58.3	303-304 bp	1.0	77.7
trnR-UCU-atpA_p3	ATCGGAATGAAAAGCGTCCA	CGGAGGCCCTTTTGAAAGAA	45.0	50.0	57.9	58.4	286-287 bp	1.0	77.2
trnR-UCU-atpA_p4	TCGGAATGAAAAGCGTCCAT	CGGAGGCCCTTTTGAAAGAA	45.0	50.0	57.9	58.4	285-286 bp	1.0	77.2
trnR-UCU-atpA_p5	TCGGAATGAAAAGCGTCCAT	AGGCCCTTTTGAAAGAAGCT	45.0	45.0	57.9	57.6	282-283 bp	1.0	77.2