Hotspot

psbE-petL

LSC · 64688-65616 · 929 bp

Back to Apium

Pi 0.0495
MarkerSeek score 83.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
929
variable_sites
43
pi
0.0495
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1087-1146 bp 1.0 86.6
psbE-petL_p2 ACTGACGAATAACCAACCCG GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1100-1159 bp 1.0 86.3
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1090-1149 bp 1.0 86.0
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.8 58.0 1010-1069 bp 1.0 86.0
psbE-petL_p5 ACTGACGAATAACCAACCCG TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1103-1162 bp 1.0 85.7