Hotspot

petA-psbJ

LSC · 62960-63912 · 953 bp

Back to Apium

Pi 0.067
MarkerSeek score 78.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
953
variable_sites
62
pi
0.067
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CAGGGCCAGAACTTCTTGTT AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1750-1764 bp 1.0 84.1
petA-psbJ_p2 CAGGGCCAGAACTTCTTGTT ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1749-1763 bp 1.0 84.1
petA-psbJ_p3 CGGATTTGGTCAGGGAGATG ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 1673-1687 bp 1.0 84.1
petA-psbJ_p4 TCAGAGCTATCGCCCCAATA AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 2084 bp 0.5 59.9
petA-psbJ_p5 TCAGAGCTATCGCCCCAATA ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 2083 bp 0.5 59.9