Hotspot

trnS-UGA-psbZ

LSC · 35588-35940 · 353 bp

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Pi 0.054
MarkerSeek score 81.20
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
353
variable_sites
19
pi
0.054
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.0 57.8 473-504 bp 1.0 81.5
trnS-UGA-psbZ_p2 GAGCTATCAACCACTCAGCC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 480-511 bp 1.0 81.2
trnS-UGA-psbZ_p3 GGAGCTATCAACCACTCAGC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 481-512 bp 1.0 81.2
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 488-519 bp 1.0 81.1
trnS-UGA-psbZ_p5 CAACCACTCAGCCATCTCTC GACCAACCATCAGGAGAAGC 55.0 55.0 58.0 58.3 475-506 bp 1.0 81.0