Hotspot

trnE-UUC-trnT-GGU

LSC · 31159-31766 · 608 bp

Back to Apium

Pi 0.202
MarkerSeek score 83.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
608
variable_sites
115
pi
0.202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GTGAATCTGTCGGGACTGAC GAACCGATGACTTACGCCTT 55.0 50.0 58.0 58.0 1097 bp 0.5 59.9
trnE-UUC-trnT-GGU_p2 CCATCTGAAAGGCGGATTCA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1345 bp 0.5 59.6
trnE-UUC-trnT-GGU_p3 AGTGAATCTGTCGGGACTGA GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 1098 bp 0.5 59.4
trnE-UUC-trnT-GGU_p4 CCATTGGGGGAGGTTATTGG GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 799 bp 0.5 59.3
trnE-UUC-trnT-GGU_p5 GTGAATCTGTCGGGACTGAC TACCGCTGAGTTAAAAGGGC 55.0 50.0 58.0 57.9 1062 bp 0.5 59.2