Hotspot

trnD-GUC-trnY-GUA

LSC · 30811-30928 · 118 bp

Back to Apium

Pi 0.5
MarkerSeek score 75.80
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
118
variable_sites
57
pi
0.5
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GTGAATCTGTCGGGACTGAC CCAATAACCTCCCCCAATGG 55.0 55.0 58.0 57.9 318 bp 0.5 59.3
trnD-GUC-trnY-GUA_p2 GTGAATCTGTCGGGACTGAC TACAATGAGCAACGGTGGAG 55.0 50.0 58.0 57.9 468 bp 0.5 59.2
trnD-GUC-trnY-GUA_p3 CCATCTGAAAGGCGGATTCA CCAATAACCTCCCCCAATGG 50.0 55.0 58.0 57.9 566 bp 0.5 59.0
trnD-GUC-trnY-GUA_p4 CCATCTGAAAGGCGGATTCA TACAATGAGCAACGGTGGAG 50.0 50.0 58.0 57.9 716 bp 0.5 58.9
trnD-GUC-trnY-GUA_p5 GTGAATCTGTCGGGACTGAC TCTTTTCTGACGCACCACTC 55.0 50.0 58.0 58.1 673 bp 0.5 58.9