Hotspot

petN-psbM

LSC · 28831-29927 · 1097 bp

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Pi 0.0505
MarkerSeek score 78.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1097
variable_sites
52
pi
0.0505
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GGCCTGGGGGTTTTTCTAAA TGAATCCGCCTTTCAGATGG 50.0 50.0 58.0 58.0 2364 bp 0.5 59.5
petN-psbM_p2 TGGCTGAGTCTTGAATTCCG TGAATCCGCCTTTCAGATGG 50.0 50.0 57.9 58.0 2240 bp 0.5 58.9
petN-psbM_p3 GGCTGAGTCTTGAATTCCGT TGAATCCGCCTTTCAGATGG 50.0 50.0 57.9 58.0 2239 bp 0.5 58.9
petN-psbM_p4 AGTCCCTTTTTGACTCTGCG TGAATCCGCCTTTCAGATGG 50.0 50.0 58.1 58.0 1848 bp 0.5 58.7
petN-psbM_p5 GGCCTGGGGGTTTTTCTAAA ATGTTTCCCAAGCGCACATA 50.0 45.0 58.0 58.2 2331 bp 0.5 58.5