Hotspot

trnC-GCA-petN

LSC · 28044-28740 · 697 bp

Back to Apium

Pi 0.0604
MarkerSeek score 68.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
697
variable_sites
16
pi
0.0604
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.38
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CGATATGGCCGAGTGGTAAG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.9 58.2 361-793 bp 1.0 84.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 321-753 bp 1.0 84.5
trnC-GCA-petN_p3 CGATATGGCCGAGTGGTAAG AGAGTCCACTTCTTCCCCAT 55.0 50.0 57.9 57.6 422-854 bp 1.0 82.7
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 382-814 bp 1.0 82.7
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 339-771 bp 1.0 82.3