Hotspot

rpoB-trnC-GCA

LSC · 27033-27972 · 940 bp

Back to Apium

Pi 0.0566
MarkerSeek score 79.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
940
variable_sites
50
pi
0.0566
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCTCATTTACATCTCGGAGC CTTACCACTCGGCCATATCG 52.4 55.0 58.2 57.9 985-1272 bp 1.0 77.1
rpoB-trnC-GCA_p2 CCCTCATTTACATCTCGGAGC CCGGATTTGAACTGGGGAAA 52.4 50.0 58.2 58.1 1025-1312 bp 1.0 76.8
rpoB-trnC-GCA_p3 CCTCATTTACATCTCGGAGCA CTTACCACTCGGCCATATCG 47.6 55.0 57.5 57.9 984-1271 bp 1.0 75.3
rpoB-trnC-GCA_p4 CCTCATTTACATCTCGGAGCA CCGGATTTGAACTGGGGAAA 47.6 50.0 57.5 58.1 1024-1311 bp 1.0 75.0
rpoB-trnC-GCA_p5 CCCTCATTTACATCTCGGAGC TTATTGATCAGGCGACACCC 52.4 50.0 58.2 57.7 1043-1330 bp 1.0 74.7