Hotspot

trnK-UUU-trnQ-UUG

LSC · 4336-7422 · 3087 bp

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Pi 0.0587
MarkerSeek score 78.40
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
3087
variable_sites
163
pi
0.0587
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT TGGTAACATAGGTCACACCCT 45.0 47.6 58.0 58.1 915-1100 bp 1.0 79.1
trnK-UUU-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT TGGTAACATAGGTCACACCCT 45.0 47.6 58.0 58.1 916-1101 bp 1.0 79.1
trnK-UUU-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TGGTAACATAGGTCACACCCT 45.0 47.6 58.1 58.1 926-1111 bp 1.0 78.3
trnK-UUU-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TGGTAACATAGGTCACACCCT 50.0 47.6 58.2 58.1 937-1122 bp 1.0 77.8
trnK-UUU-trnQ-UUG_p5 GGGGTTTTTCGTACCGATCA TGGTAACATAGGTCACACCCT 50.0 47.6 57.9 58.1 635 bp 0.5 50.5