Hotspot

psaC

SSC · 113196-113441 · 246 bp

Pi 0.496

MarkerSeek score 76.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 113
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TTTCTTTCCACCCCACATGG	ATCGGACCCGCTATTGTTTC	50.0	50.0	58.0	58.1	1238 bp	0.5	59.5
psaC_p2	AAAACAGAACTTCCCGCAGT	ATCGGACCCGCTATTGTTTC	45.0	50.0	57.9	58.1	1291 bp	0.5	59.2
psaC_p3	CGGTTGAGCTGCTAAGGTAG	ATCGGACCCGCTATTGTTTC	55.0	50.0	58.1	58.1	1079 bp	0.5	59.0
psaC_p4	TTTCTTTCCACCCCACATGG	CTATGGGTTGATCACGAGCC	50.0	55.0	58.0	58.1	1410 bp	0.5	58.9
psaC_p5	GTAACCGGTTGAGCTGCTAA	ATCGGACCCGCTATTGTTTC	50.0	50.0	57.9	58.1	1084 bp	0.5	58.9