Hotspot

ndhI

SSC · 115273-115758 · 486 bp

Pi 0.474

MarkerSeek score 78.20

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 194
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AACATTAATAGCGCCCACGT	TGATTTACCGGAAGCGGAAG	45.0	50.0	58.0	58.0	1644 bp	0.5	59.6
ndhI_p2	AATCATGTAGTGGTTCGGGC	TGATTTACCGGAAGCGGAAG	50.0	50.0	58.0	58.0	1456 bp	0.5	59.5
ndhI_p3	AACATTAATAGCGCCCACGT	TTTACCGGAAGCGGAAGAAG	45.0	50.0	58.0	57.9	1641 bp	0.5	59.0
ndhI_p4	AATCATGTAGTGGTTCGGGC	TTTACCGGAAGCGGAAGAAG	50.0	50.0	58.0	57.9	1453 bp	0.5	59.0
ndhI_p5	AACATTAATAGCGCCCACGT	GGCCCGGTCCTTAGATTTTT	45.0	50.0	58.0	57.9	1894 bp	0.5	58.6