Hotspot

ndhH

SSC · 118165-119346 · 1182 bp

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Pi 0.462
MarkerSeek score 79.30
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
434
pi
0.462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TCAGGTCCAATACGCTGTTG GAAAACCCAGGATCGGTTGA 50.0 50.0 57.9 57.8 1701 bp 0.5 57.7
ndhH_p2 TCAGGTCCAATACGCTGTTG AGAAAACCCAGGATCGGTTG 50.0 50.0 57.9 57.8 1702 bp 0.5 57.7
ndhH_p3 CAACGAATCGCACGTAGAGA GAAAACCCAGGATCGGTTGA 50.0 50.0 58.1 57.8 2073 bp 0.5 57.6
ndhH_p4 CTCAACGAATCGCACGTAGA GAAAACCCAGGATCGGTTGA 50.0 50.0 58.1 57.8 2075 bp 0.5 57.6
ndhH_p5 TCAACGAATCGCACGTAGAG GAAAACCCAGGATCGGTTGA 50.0 50.0 58.1 57.8 2074 bp 0.5 57.6