Hotspot

ndhG

SSC · 114281-114811 · 531 bp

Pi 0.477

MarkerSeek score 78.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 203
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GAAACAATAGCGGGTCCGAT	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.1	57.9	1541 bp	0.5	58.8
ndhG_p2	GAAACAATAGCGGGTCCGAT	CGACTTATGATCGCCACGAA	50.0	50.0	58.1	58.2	1570 bp	0.5	58.3
ndhG_p3	GAAACAATAGCGGGTCCGAT	TTCGACTTATGATCGCCACG	50.0	50.0	58.1	58.2	1572 bp	0.5	58.3
ndhG_p4	GGCTCGTGATCAACCCATAG	AATCAAATTGCTTTGGGCCG	55.0	45.0	58.1	57.9	1369 bp	0.5	58.2
ndhG_p5	GGCTCGTGATCAACCCATAG	CGACTTATGATCGCCACGAA	55.0	50.0	58.1	58.2	1398 bp	0.5	57.6