Hotspot

ndhF

SSC · 106758-109013 · 2256 bp

Back to Apios

Pi 0.435
MarkerSeek score 78.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2256
variable_sites
818
pi
0.435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCGGCAATAATCACAAAAACGT GTACGCTTTTTGGGAACTGC 36.4 50.0 57.5 58.0 2941 bp 0.5 39.7
ndhF_p2 CGGCAATAATCACAAAAACGT GTACGCTTTTTGGGAACTGC 38.1 50.0 56.1 58.0 2940 bp 0.5 36.2
ndhF_p3 CAGTTCTTCGGCAATAATCACA GTACGCTTTTTGGGAACTGC 40.9 50.0 57.1 58.0 2948 bp 0.5 36.1
ndhF_p4 AACTGTCATAGCTCTACCCT GTACGCTTTTTGGGAACTGC 45.0 50.0 54.9 58.0 2979 bp 0.5 35.2
ndhF_p5 TTCGGCAATAATCACAAAAACGT GTACGCTTTTTGGGAACTGC 34.8 50.0 58.1 58.0 2942 bp 0.5 34.5