Hotspot

ndhE

SSC · 113746-114051 · 306 bp

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Pi 0.466
MarkerSeek score 72.80
Primer available yes
Label ndhE

Feature Metadata

label_name
ndhE
feature_type
gene
region
SSC
length_bp
306
variable_sites
111
pi
0.466
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE_p1 TCTTGGGGCAGAAGCAATTT TGTCGTGACTCGCCAATAAG 45.0 50.0 58.0 58.0 990 bp 0.5 59.8
ndhE_p2 TTCTTGGGGCAGAAGCAATT TGTCGTGACTCGCCAATAAG 45.0 50.0 58.0 58.0 991 bp 0.5 59.8
ndhE_p3 ACCCATGCTTCGAGTTGTTT TGTCGTGACTCGCCAATAAG 45.0 50.0 58.0 58.0 1092 bp 0.5 59.8
ndhE_p4 GCTTCGAGTTGTTTCATGCC TGTCGTGACTCGCCAATAAG 50.0 50.0 58.0 58.0 1086 bp 0.5 59.7
ndhE_p5 TCTTGGGGCAGAAGCAATTT ACGTGGGCGCTATTAATGTT 45.0 45.0 58.0 58.0 1306 bp 0.5 59.6