Hotspot

ndhD

SSC · 111569-113068 · 1500 bp

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Pi 0.443
MarkerSeek score 81.50
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1500
variable_sites
580
pi
0.443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GATCGTTGGGGTTACCGTAC AAATTGCTTCTGCCCCAAGA 55.0 45.0 58.1 58.0 2340 bp 0.5 59.3
ndhD_p2 GATCGTTGGGGTTACCGTAC AATTGCTTCTGCCCCAAGAA 55.0 45.0 58.1 58.0 2339 bp 0.5 59.3
ndhD_p3 GATCGTTGGGGTTACCGTAC AAACAACTCGAAGCATGGGT 55.0 45.0 58.1 58.0 2238 bp 0.5 59.3
ndhD_p4 GATCGTTGGGGTTACCGTAC GGCATGAAACAACTCGAAGC 55.0 50.0 58.1 58.0 2244 bp 0.5 59.2
ndhD_p5 CTAATGAGGCATGGGGATCG AATTGCTTCTGCCCCAAGAA 55.0 45.0 57.9 58.0 2263 bp 0.5 59.0