Hotspot

ndhA

SSC · 115840-118163 · 2324 bp

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Pi 0.458
MarkerSeek score 79.00
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2324
variable_sites
900
pi
0.458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 CGGCCCAAAGCAATTTGATT TTCGTCCACCGGGTTTTATC 45.0 50.0 57.9 57.9 2959 bp 0.5 58.4
ndhA_p2 CGTGGCGATCATAAGTCGAA TTCGTCCACCGGGTTTTATC 50.0 50.0 58.2 57.9 2928 bp 0.5 57.9
ndhA_p3 TTCGTGGCGATCATAAGTCG TTCGTCCACCGGGTTTTATC 50.0 50.0 58.2 57.9 2930 bp 0.5 57.9
ndhA_p4 ACCAATGTACCTTGCAGCTC TTCGTCCACCGGGTTTTATC 50.0 50.0 58.2 57.9 2597 bp 0.5 57.8
ndhA_p5 GACCAATGTACCTTGCAGCT TTCGTCCACCGGGTTTTATC 50.0 50.0 58.2 57.9 2598 bp 0.5 57.8