Hotspot

ccsA

SSC · 110318-111295 · 978 bp

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Pi 0.468
MarkerSeek score 82.60
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
978
variable_sites
417
pi
0.468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TAGACACGCTGCTCTTAGGA TTTCGGCTGACAAGGTTGAA 50.0 45.0 57.9 58.0 1446 bp 0.5 58.6
ccsA_p2 TAGACACGCTGCTCTTAGGA TTTTCGGCTGACAAGGTTGA 50.0 45.0 57.9 58.0 1447 bp 0.5 58.6
ccsA_p3 AAATTGGTAGACACGCTGCT TTTCGGCTGACAAGGTTGAA 45.0 45.0 57.8 58.0 1453 bp 0.5 58.2
ccsA_p4 AAATTGGTAGACACGCTGCT TTTTCGGCTGACAAGGTTGA 45.0 45.0 57.8 58.0 1454 bp 0.5 58.2
ccsA_p5 GTAGACACGCTGCTCTTAGG TTTCGGCTGACAAGGTTGAA 55.0 45.0 57.8 58.0 1447 bp 0.5 58.1